Journal of Engineering Sciences / Журнал інженерних наук
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Item Decontamination of oil-polluted soils: Power of electronic bioinformatic databases(Sumy State University, 2022) Аблєєва, Ірина Юріївна; Аблеева, Ирина Юрьевна; Ablieieva, Iryna Yuriivna; Пляцук, Леонід Дмитрович; Пляцук, Леонид Дмитриевич; Pliatsuk, Leonid Dmytrovych; Liu, T.; Бережна, Ірина Олексіївна; Бережная, Ирина Алексеевна; Berezhna, Iryna Oleksiivna; Янченко, Ілона Олегівна; Янченко, Илона Олеговна; Yanchenko, Ilona OlehivnaThe main idea was to solve the problem related to oil contamination of soil using bioremediation with bioaugmentation with modeled microorganism strains. The paper aimed to develop a bacterial consortium for petroleum hydrocarbon degradation during the biological treatment of oil-contaminated soils using electronic databases. The research methodology included an analysis of the mechanisms and metabolic pathways of petroleum hydrocarbon degradation and an assessment of the possible reaction modulus and enzymatic systems for the degradation of aromatic compounds. The taxonomic classification and review of oil compound transformation metabolic pathways were carried out using electronic KEGG, MetaCyc, and EzTaxon databases. The KEGG database was used to create a microbiological consortium of certain strains of bacteria that improved hydrocarbon degradation process performance. Identification of bacteria’s complete genome using Island Viewer 4 allowed to create of a consortium of oil-destructive bacteria consisting of such strains: Pseudoxanthomonas spadix BD-a59, Rhodococcus jostii RHA1, Rhodococcus aetherivorans IcdP1, Pseudomonas putida ND6, Pseudomonas stutzeri 19SMN4, Pseudomonas fluorescens UK4, Acinetobacter lactucae OTEC-02, Bacillus cereus F837/76.7.9. The ratio between the mentioned strains of microorganisms in the consortium was set at 20 % : 20 % : 15 % : 10 % : 10 % : 5 % : 5 % : 15 %. This bacterial consortium for aromatic hydrocarbons was created according to the metabolic information of basic enzymatic systems and the predominant transformation of particular oil compounds using the BacDive database.Item Digestate potential to substitute mineral fertilizers: Engineering approaches(Sumy State University, 2022) Аблєєва, Ірина Юріївна; Аблеева, Ирина Юрьевна; Ablieieva, Iryna Yuriivna; Geletukha, G.G.; Kucheruk, P.P.; Enrich-Prast, A.; Carraro, G.; Бережна, Ірина Олексіївна; Бережная, Ирина Алексеевна; Berezhna, Iryna Oleksiivna; Бережний, Дмитрій Михайлович; Бережной, Дмитрий Михайлович; Berezhnyi, Dmytrii MykhailovychThe study aims to define the potential and technological aspects of the digestate treatment for its application as a biofertilizer. Life cycle assessment methodology was used in terms of digestate quality management. The potential of nutrients, organic carbon, and useful microelements in the digestate allows for its consideration as a mineral fertilizer substitute and soil improver. The valorization of digestate as fertilizer requires quality management and quality control. Based on the research focus, the successful soil application of digestate post-treatment technologies was analyzed. Among the different commercial options for digestate treatment and nutrient recovery, the most relevant are drying, struvite precipitation, stripping, evaporation, and membranes technology. Comparing the physical and chemical properties of the whole digestate, separated liquid, and solid liquor fractions showed that in the case of soil application of granular fertilizer, nutrients from the digestate are released more slowly than digestate application without granulation. However, realizing this potential in an economically feasible way requires improving the quality of digestate products through appropriate technologies and quality control of digestate products. To support the manufacture of quality digestate across Europe, the European Compost Network developed a concept for a panEuropean quality assurance scheme.